Abstract: The effect of ECT combinated by different voltage and electric quantity on killer rate of Human cervical carcinoma Hela-S3 colon. proliferating rate of cells, karyokinesis inde and cells proliferating period was observed by MTT methed and autor adiography. The purpose of the experiments is to find out the effect of ECT on the bilologi-cal act of Hela-S3 colon.The results showed ECT has the following action: (1)Ob-vious inhibition action on the proliferating of Hela-S3 colon.The inhibiting rate of 5V10C is the most obvious one. (2)Killer action on Hela-S3 colon.It has direct rate with electric quatity under the same vloltage. (3)Obvious effect on the cells proliferating period, mainly on GI-S period and M period and observed, and it is re-lated with voltage,electric quantiry. The above results showed ECT has obvious effect on the bilogioal behavior. The results also contribute to the treatment of human cervical carcinoma, they play an important part when doctors are selecting effective ways of treating patients.