Abstract: Objective To explore the significance and role of P⁵³gene transfer on human glioma cell growth in nude mice. Method Wild-type P⁵³ gene(wt-P⁵³)and mutant P⁵³ gene(mt-P⁵³)were transfected into the human glioma cell line SHG44.And the growth of gene-transfected cell lines were observed in vitro and in vivo. Results The tumor resulting from the cells transfected with the wt-P⁵³ gene more slowly and was smaller than that from control SHG44 cells.In contrast,the tumor from the ce-lls transfected with the mt-P⁵³ gene grew faster than that produced by cells transfected with the Wt-P⁵³ gene and that produced by control SHG44 cells. Conclusion The wild-type P⁵³ gene could inhibit the glioma cell growth in nude mice and the mutant P⁵³ gene could enhance the glioma cell growth in nude face.