VEGF受体flk-1的表达与喉粘膜上皮癌变发生和转移的关系
Relationship between Expression of Vascular Endothelial Growth Factor Receptor, flk-1 and the Carcinogenesis and Metastases of Laryngeal Mucosa
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摘要: 目的:观察血管内皮生长因子(VEGF)受体flk－1在不典型增生及鳞癌中的表达,用图象分析系统定量的方法观察flk-1与喉癌的生长、浸润及转移的关系。方法:用抗flk－1抗体,在经过微波处理组织暴露抗原后,进行标准化的EliteABC染色,并通过图象分析系统确定flk-1在不典型增生、鳞癌中的表达。结果:在不典型增生及鳞癌中flk－1均有表达。在组织中,flk－1不仅在血管内皮细胞中、在部分浆细胞和巨噬细胞中也呈阳性反应。鳞癌组中的flk－1的表达明显高于不典型增生组(P<0.05)。在鳞癌中,随着分化程度的下降,flk-1表达有逐渐递增的趋势。在鳞癌组中发生转移的病变flk-1的表达明显高于未转移组(P<0.05)。相关分析显示flk－1的表达与肿瘤的临床分期密切相关。结论:VEGF通过自分泌和旁分泌的途径影响肿瘤细胞生长及间质中新生血管的形成,肿瘤细胞的生长与血管的发生相互依赖。flk-1与喉癌的生长、浸润及转移密切相关,flk-1可能作为一个判断预后的重要指标。Abstract: Using automated image analysis to quantify the immunoreactivities of flk-1 in dysplasia (DYS) and malignant epithelia (SCC) of laryngeal mucosa, to observe the distributton of flk-1 and the relationship between flk-1 and the growth, infiltration andmetastases of laryngeal carcinoma. Results showed that flk-1 was expressed by the epithelial cell in all observed groups.The endothelial cells and the chronic inflammatorycells were also flk-1-positive stained.The expression of flk-1 was higher in SCC than inDYS.The expression of flk-l in metastatic SCC was significantly higher than in theSCC without metastases.Correlation analysis indicated that expression of flk-l washighly correlated with the clinical stage.We concluded that VEGF could influence thegrowth of both tumour cells and newly foumed blood vessels through paracrine and autocrine patterns.The expression of flk-l was highly associated with the growth, infiltration and metastases of laryngeal carcinoma, which might be an important prognosticindicator for laryngeal carcinoma.
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