SRRS方剂抑制消化道恶性肿瘤P53基因突变的实验研究
The Effect of SRRS Prenventing P 53 Gene from Mutation
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摘要: 目的：从分子水平探讨SRRS方剂治疗消化道癌的可能机制。方法：选用Lovo结肠腺癌细胞裸小鼠移植瘤模型，观察中药SRRS方剂抑制移植瘤生长的作用，并用PCR-SSCP银染色法研究SRRS方剂对Lovo移植瘤生长过程中P53基因突变的影响情况。结果：对照组有50%(5/10)发生P53基因第七外显子突变，SRRS方剂治疗组无(0/10)此现象(P<0.05)。结论：提示SSRS方剂能抑制lovo移植瘤生长过程中P53基因的突变，即SRRS方剂可对肿瘤生长过程中肿瘤细胞的基因水平有影响作用。Abstract: In order to study the mechanism of the tumor suppressor effect of the “SRRS”,a recipe composed of some Chinese herbs,the mutation of P 53 gene exon 7 in the tumor tissue was observed by PCR SSCP in the nude mice Lovo transplantation tumors.The important observation of this study was that none of the samples(0/10)was shown to have P 53 gene exon7 mutation in the experimental group which SRRS was taken.After all,50%(5/10) of the samples were shown to have P 53 mutation in the control group.This result suggested that SRRS could prevent P 53 gene from mutating during the Lovo transplatstion tumors growing.
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