银染-TRAP法检测端粒酶活性的建立
Detection of Telomerase Activity by Sliver Staining Telomeric Repeat Amplification Protocol
-
摘要: 目的: 建立检测端粒酶活性的改良银染TRAP法（端粒重复序列扩增法)。方法: 用银染TRAP法测定人肿瘤细胞株和经加热处理的阴性对照细胞的端粒酶活性。结果: 肿瘤细胞株的端粒酶活性阳性率为100%, 10、100、500个细胞均为阳性, 加热处理后的细胞端粒酶活性均为阴性。结论: 提示与核素TRAP法相比较, 银染TRAP法是同样特异、敏感, 且更快速的端粒酶活性的检测方法, 该方法可成为恶性肿瘤的诊断指标之一。Abstract: To detect the telomerase activity in human tumor cell line, we established the new method of sliver staining Telomeric Repeat Amplification Protocol(TRAP). We detected the telomerase activity in human tumor cell line and heat-treated cells as control by combining the sliver stain with TRAP. The human tumor cells were positive for the telomerase activity and the heat-treated cells were negative. Comparing with radioisotopic TRAP, sliver staining TRAP was specific, sensitive, harmless and fast assay for telomerase activity. It could be one of the marks in cancer.
下载:
