人胃癌细胞系SGC-7901放射敏感性及异质性的研究

Study about the Radiosensitivity and Heterogeneity of Human Gastric Cancer Cell Line (SGC-7901)

摘要: 目的: 研究人胃癌细胞系SGC-7901及其克隆亚系的放射敏感性。方法: SGC-7901细胞株及其两个克隆亚系（大集落、小集落）的细胞用8MeV直线加速器电子束以不同剂量照射, 分别经48小时、72小时培养及集落培养后, 计数并得出各组存活曲线。结果: 1.两个克隆亚系之间的D₀值有显著差异(P<0.05)大集落亚系与SGC母系之间在集落形成实验中, 其D₀值有差异（P<0.05）。结论: SGC-7901细胞系及其克隆亚系对放射的敏感性存在着异质性。

Abstract: Objective: To study the radiosensitivity of human gastric cancer cell line(SGC-7901)and two subcell lines picked from the line. Methods: The cells of the cell line and two subcell lines (large clone and small clone) were radioted at different doses with 8MeV linear accelerator. Then the cells were incubated for 48h, 72h and clone culture. The survival curve was established by counting cell. Results: (1)The values of Do between two subcell lines were significantly different(P <0.05); (2)the values of Do between large clone subcell line and SGC-7901 cell line were significantly different (P <0.05). Conclusion: Heterogeneity of the radiosensitivity among SGC-7901 and the subcell lines is suggested.