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EGCG对鼻咽癌细胞增殖、凋亡及E2F-1表达的影响

李彬彬, 黄培春, 黄国良, 何志巍

李彬彬, 黄培春, 黄国良, 何志巍. EGCG对鼻咽癌细胞增殖、凋亡及E2F-1表达的影响[J]. 肿瘤防治研究, 2012, 39(12): 1407-1410. DOI: 10.3971/j.issn.1000-8578.2012.12.001
引用本文: 李彬彬, 黄培春, 黄国良, 何志巍. EGCG对鼻咽癌细胞增殖、凋亡及E2F-1表达的影响[J]. 肿瘤防治研究, 2012, 39(12): 1407-1410. DOI: 10.3971/j.issn.1000-8578.2012.12.001
Li Binbin, Huang Peichun, Huang Guoliang, He Zhiwei. Effect of EGCG on Proliferation,Apoptosis and E2F-1 Expression of Nasopharyngeal Carcinoma Cell[J]. Cancer Research on Prevention and Treatment, 2012, 39(12): 1407-1410. DOI: 10.3971/j.issn.1000-8578.2012.12.001
Citation: Li Binbin, Huang Peichun, Huang Guoliang, He Zhiwei. Effect of EGCG on Proliferation,Apoptosis and E2F-1 Expression of Nasopharyngeal Carcinoma Cell[J]. Cancer Research on Prevention and Treatment, 2012, 39(12): 1407-1410. DOI: 10.3971/j.issn.1000-8578.2012.12.001

EGCG对鼻咽癌细胞增殖、凋亡及E2F-1表达的影响

基金项目: 广东省中医药管理局资助项目(81072059)
详细信息
    作者简介:

    李彬彬(1976-),女,硕士,副教授,主要从事鼻咽癌的防治研究

    通信作者:

    何志巍,E-mail:467853605@qq.com

  • 中图分类号: R739.63;R730.231

Effect of EGCG on Proliferation,Apoptosis and E2F-1 Expression of Nasopharyngeal Carcinoma Cell

  • 摘要: 目的 研究表没食子儿茶素没食子酸酯(EGCG)对鼻咽癌细胞增殖和凋亡的影响及其分子机制。方法采用CCK-8法检测EGCG对鼻咽癌细胞株CNE-2Z增殖的影响;AnnexinV /PI双标记流式细胞术检测EGCG的凋亡诱导作用;实时荧光定量PCR和Western blot检测核转录因子E2F-1及其上游调控蛋白CyclinD1和 p21的表达水平。结果EGCG处理后,CNE-2Z细胞的增殖明显受到抑制,其细胞凋亡率亦显著增高,呈量效关系;随着EGCG浓度增高,E2F-1 mRNA和蛋白表达水平明显下降,其上游调控蛋白CyclinD1的表达亦明显下调,而p21的表达则明显上调。结论EGCG可明显抑制鼻咽癌细胞增殖和诱导细胞凋亡,可能与其直接或间接下调核转录因子E2F-1有关,CyclinD1和p21参与其调控。

     

    Abstract: Objective To study the effects of epigallocatechin-3-gallate(EGCG)on proliferation and apoptosis of nasopharyngeal carcinoma cell and its mechanism. Methods The growth and proliferation of CNE-2Z cell was detected by CCK-8 assay,and apoptosis was assessed by AnnexinV/PI flow cytometry.The expressions of nuclear transcription factor E2F-1 and its regulatory protein CyclinD1 and p21 were detected by real-time quantitative PCR and Western blot. Results The proliferation of CNE-2Z cell was inhibited in a dose-dependent manner after treatment with EGCG,the apoptosis was induced as well.The expression of E2F-1 mRNA and protein in CNE-2Z cell were markedly suppressed by EGCG,and the level of its regulatory protein CyclinD1 also decreased while p21 was increased significantly. Conclusion EGCG suppressed the proliferation and induced apoptosis in CNE-2Z cell by directly or indirectly down-regulating E2F-1,which was regulated by CyclinD1 and p21.

     

  • [1] Lang JY,Li T,Lin B.Current status and prospect on multimodality therapy for nasopharyngeal carcinoma[J].Zhongguo Zhong Liu,2006,15(12):826-31.[郎锦义,李涛,林冰.鼻咽癌综合治疗研究现状与展望[J].中国肿瘤,2006,15(12):826-31.]
    [2] Ju J,Lu G,Lambert JD,et al.Inhibition of carcinogenesis by tea constituents[J].Semin Cancer Biol,2007,17(5):395-402.
    [3] Johnson DG,Schwarz JK,Cress WD,et al.Expression of transcription factor E2F1 induces quiescent cells to enter S phase [J].Nature,1993,365(6444):349-52.
    [4] Zhang SY,Liu SC,Al-Saleem LF,et al.E2F-1:a proliferative marker of breast neoplasia[J].Cancer Epidemiol Biomarkers Prev,2000,9(4):395-401.
    [5] Ebihara Y,Miyamoto M,Shichinohe T,et al.Over-expression of E2F-1 in esophageal squamous cell carcinoma correlates with tumor progression[J].Dis Esophagus,2004,17(2):150-4.
    [6] Zacharatos P,Kotsinas A,Evangelou K,et al.Distinct expression patterns of the transcription factor E2F-1 in relation to tumour growth parameters in common human carcinomas[J].J Pathol,2004,203(3):744-53.
    [7] Maehara K,Yamakoshi K,Ohtani N,et al.Reduction of total E2F/DP activity induces senescence-like cell cycle arrest in cancer cells lacking functional pRB and p53[J].J Cell Biol,2005,168(4):553-60.
    [8] Xiao Q,Li L,Xie Y,et al.Transcription factor E2F-1 is upregulated in human gastric cancer tissues and its overexpression suppresses gastric tumor cell proliferation[J].Cell Oncol,2007,29(4):335-49.
    [9] Sun A,Bagella L,Tutton S,et al.From G0 to S phase:a view of the roles played by the retinoblastoma(Rb)family members in the Rb-E2F pathway[J].J Cell Biochem,2007,102(6):1400-4.
    [10] Shimizu M,Deguchi A,Lim JT,et al.(–)-Epigallocatechin gallate and polyphenon E inhibit growth and activation of the epidermal growth factor receptor and human epidermal growth factor receptor-2 signaling pathways in human colon cancer cells[J].Clin Cancer Res,2005,11(7):2735-46.
    [11] Zhao Y,Luo FJ,Tang FQ,et al.Interference of epigallocatechin-3- gallate on targets of nuclear factor κB signal transduction pathway activated by EB virus encoded latent membrane protein 1[J].Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan,2003,30(1):214-9.[赵燕,罗非君,唐发清,等.GCG干预LMP1激活的NF-κB信号转导通路中的靶分子[J].生物化学与生物物理进展,2003,30(1):214-9.]
    [12] Nihal M,Ahmad N,Mukhtar H,et al.Anti-proliferative and proapoptotic effects of(-)-epigallocatechin-3-gallate on human melanoma:possible implications for the chemoprevention of melanoma[J].Int J Cancer,2005,114(4):513-21.
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出版历程
  • 收稿日期:  2012-01-15
  • 修回日期:  2012-05-06
  • 刊出日期:  2012-12-24

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