重组腺病毒介导的人野生型p53、GM-CSF和B7-1基因在肝癌细胞中的表达
Wild-type p53,GM-CSF,B7-1 genes transduced with recombinant adenovirus expression in hepatoma cell lines in vitro
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摘要: 目的 观察腺病毒载体对肝癌细胞的转染效率及其介导的人野生型 p5 3、GM CSF和B7 1基因在肝癌细胞中的表达。方法 不同MOI的Ad GFP感染肝癌细胞, 48h后计算感染效率 ;BB 1 0 2以5 0MOI感染肝癌细胞, 48h后免疫组化法及westernblot检测 p5 3表达, ELISA检测GM CSF的含量, FACS测定B7 1的表达。结果 MOI为 5 0 pfu/细胞时对肝癌细胞的转染效率达 80 %以上, 目的基因均可在肝癌细胞中高效表达。结论 腺病毒载体对肝癌细胞具有较高的转染效率, 目的基因均可在肝癌细胞中高效表达, 为进一步研究BB 1 0 2在肝癌治疗中的应用奠定了基础Abstract: Objective To evaluate the transferring efficiency of recombinant adenoviral vector(BB-102) expressing the human wild type p53,GM-CSF and B7-1 genes into hepatocellular carcinoma(HCC) cell lines in vitro. Methods HCC cell lines were transferred with mock adenoviral vector expressing green fluorescent protein(Ad-GFP) at a series concentrations of MOI. p53, GM-CSF, and B7-1 expressing in HCC cells were determined by western blot, ELISA method and flow-cytometric analyses respectively. Results When 50...
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