端粒酶活性检测是鉴别良、恶性胸水的重要指标
Detection of telomerase activity is an important index for differential diagnosis between benign and malignant pleural effusion
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摘要: 目的 检测胸水脱落细胞端粒酶活性, 为临床鉴别良恶性胸水提供诊断依据。方法 离心收集各种类型胸水的脱落细胞, TRAP PCR -ELISA银染法检测脱落细胞端粒酶活性。结果 心力衰竭、肝硬化、尿毒症、肺炎、结核性胸膜炎胸水脱落细胞不能检出端粒酶活性, 而癌性胸水 91 .42 %端粒酶阳性, 端粒酶活性检测对恶性胸水诊断的敏感性为 91 .42 %, 特异性为 1 0 0 %, 显著高于其他检测方法。结论 端粒酶活性检测可作为临床良恶性胸水鉴别诊断的依据之一。Abstract: Objective To detect the telomerase activity of cells in pleural effusionecl to provide scientific basis for differential diagnosis. Methods Harvesting the cells in all kinds of pleural effusionecl and detect the telomerase activity by TRAP-ELISA. Results Cells in pleural effusion of heart failure, hepatic cirrhosis, chronic renal failure, pneumonia and tuberculosis cannot be detected for telomerase activity, for malignant pleural effusion,91.42% were telomerase positive. The sensitivity of telomerase ac...
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