阳离子脂质体介导HSV-TK/ACV系统对人脑胶质瘤细胞增殖活性的影响
Effects of the proliferation activity of human glioma cells with HSV-TK/ACV system mediated by cationic liposome
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摘要: 目的 探讨HSV TK/ACV系统对人脑胶质瘤细胞增殖活性的影响。方法 用阳离子脂质体Lipofectamine将 pCR3 Uni及含HSV TK基因的真核表达载体 pCR3 TK转染至人脑胶质瘤细胞株TJ90 5中, 筛选出阳性克隆, 阳性细胞克隆给予ACV(5 0mg/ml), 72小时后, 收集玻片, 进行AgNOR染色。 结果 转染HSV TK基因的细胞, 在给予ACV后, 细胞增殖活性明显降低, 转染 pCR3 Uni和 pCR3 -TK细胞克隆的AgNOR颗粒数分别为 1 4.3 3和 6.67(P <0 .0 1 )。结论 AgNOR计数是一种操作简便、检测细胞增殖活性的方法, 为研究HSV TK/ACV系统的抗肿瘤机制提供帮助Abstract: Objective To study the effect of the proliferation activity of human glioma cells with HSV-TK/ACV system. Methods The eukaryotic expressing vectors pCR3-TK containing the HSV-TK gene and pCR3-Uni were transferred into TJ905 cells with cationic liposome, Lipofectamine .After transfection,G418 was used to select the positive clones. The psoitive clones were given ACV(50μg/ml) and collected afer 72 hours. The glass slides were stained with silver. Results The number of silver stained AgNORs in the cell cl...
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