遗传学和免疫表型分析在急性粒单核细胞白血病临床诊断中的意义
Significance of Genetical and Immunological Analysis in Acute Myelomonocytic Leukemia for Clinical Diagnosis
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摘要: 目的 探讨染色体G显带、荧光原位杂交和流式细胞术在急性粒单核细胞白血病临床诊断、鉴别诊断和预后中的意义。方法 运用染色体G显带和流式细胞术, 对 15例骨髓细胞形态学拟诊为急性髓系白血病M4型 (AML M4 )的患者进行核型分析和免疫分型, 并用间期荧光原位杂交技术 (inter phasalfluorescenceinsituhybridization, I FISH)对其中 6例患者进行检测。结果 染色体G显带核型分析显示正常核型 6例, 伴有inv(16 ) (p13;q2 2 )异常 2例, 伴有del(16 ) (q2 2 )、 X, 5 q, t(8;2 1) (q2 2 ;q2 2 )、t(9 ;2 2 ) (q34;q11)、t(11;17)和t(11;?)异常各 1例, 另有 2例无分裂相可供分析。I FISH对 3例患者进行CBFβ基因重排检测, 2例阳性, 1例阴性 ;对这例阴性患者进一步检测AML1/ETO融合基因, 结果阳性 ;3例患者检测了MLL基因重排, 2例阳性, 1例阴性但伴有t(9;2 2 )异常, BCR/ABL融合基因阳性。免疫分型提示 14例患者有髓系和单核系分化抗...Abstract: Objective To elucidate the significance of chromosomal G banding, interphasal fluorescence in situ hybridization(I FISH) and flow cytometry for the clinical diagnosis, differentiation and prognosis estimation in acute myelomonocytic leukemia. Methods Chromosomal G banding and flow cytometry were used for 15 cases who were initially diagnosed the M4 subtype from acute myeloid leukemia (AML M4) by morphology, including 6 cases differentiated by I FISH. Results Karyotypes of 15 cases were analyzed, 6 nor...
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