VEGF反义核酸与柔红霉素联用对增加髓性白血病细胞化疗敏感性的研究
Study of VEGF Antisense Oligodeoxynudeotide and Daunorubicin to Increase the Sensitivity of AML and CML Cells
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摘要: 目的 探讨血管内皮生长因子VEGF(VascularEndothelialGrowthFactor)反义核酸与柔红霉素联用, 对AML、CML细胞药物敏感性的影响。方法 采用以前实验筛选所得的最优反义核酸A7,长度为 2 0脱氧核糖核苷酸, 全硫代修饰 ;以脂质体介导转染细胞, 2 4h以后加入柔红霉素, 继续培养 72h, 用0 .4 %苔盼蓝拒染法计数活细胞, 用ELISA法检测培养液中VEGF蛋白的浓度, 用流式细胞仪检测细胞凋亡百分数。结果 VEGF反义核酸显著增加柔红霉素抑制AML、CML细胞生长, 下调VEGF蛋白的表达, 诱导AML、CML细胞凋亡。结论 VEGF反义核酸提高AML、CML细胞对柔红霉素敏感性 ;内源性VEGF蛋白使AML、CML细胞产生耐药性Abstract: Objective To explore the effect of VEGF antisense on sensitivity of AML and CML cells to Daunorubicin (DNR). Methods A7, which was the most effective one of AS PS ODN selected with help design of computer and experimental assay, contains 20 mer modified with phosprothioate and was transferred into cells mediated with lipofectin. 24 hours later, DNA was added into culture system for continuing incubation for 72h. And then the viable cells were detected with trypan blue exclusion every 24 hours, cell ap...
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