白血病和淋巴瘤常见染色体融合基因的检测分析

Detection and Analysis of Common Fusion Genes Related to Leukemias and Lymphomas

摘要: 目的　应用多重巢式RT PCR法, 结合染色体核型检查分析 75例不同类型白血病观察其治疗和预后。方法　收集 4 1例AML、8例CML、1例CLL、1例PLL、6例MDS、8例NHL、4例MM、5例ALL及 1例嗜酸细胞性白血病患者骨髓标本进行RT PCR及染色体核型检查。结果　 (1)RT PCR示 :75例白血病中 30例阳性条带, 4 5例无阳性条带。其中M 3:PML/RARA的阳性率为 5 0 %, PLZF/RARA 8.3%, (包括 3/ 12例门诊复查病例, 2 / 12例为同一患者 )。M 4 :CBFB/MYH114 4 .4 %, MLLex7/MLLex2 11.1%。M5 :MLL/AF6 33.3%。CML :BCR/ABL 87.5 %。MDS :MLLex7/AF4 16 .7%, EVI1(3q2 6 ) 16 .7%。NHL :EVI1(3q2 6 ) 12 .5 %。MM :HOX115 0 .0 %。ALL :BCR/ABL 2 0 .0 %。 (2 )染色体核型检查多数与融合基因PCR结果相符, 2例染色体核型检查正常而融合基因检测有阳性条带。结论　 (1)某些融...

Abstract: Objective To detect common fusion genes in 75 cases of leukemias and lymphomas by both nested multiplex RT PCR and chromosome karyotype. Methods Bone marrow samples from 75 patients, including 41 acute myelogenous leukemia (AML), 8 chronic myelogenous leukemia (CML), 1 chronic lymphoblastic leukemia (CLL), 1 prolymphocytic leukemia (PLL), 6 myelodysplastic syndrome (MDS), 8 non Hodgkin s lymphoma (NHL), 4 multiple myeloma (MM), 5 acute lymphoblastic leukemia (ALL), and 1 eosinophilic leukemia, were anal...