血小板源性生长因子对肿瘤细胞体外作用的初步研究
Effect of PDGF on the in-Vitro Growth of Leukemic Cell Dami
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摘要: 目的 本文试图了解PDGF对肿瘤细胞体外生长和胞内传递通路的作用及肿瘤细胞是否表达PDGF受体。方法 应用白血病细胞株Dami作为肿瘤细胞模型, 观察PDGF对Dami细胞的体外增殖和集落生成的影响, 并应用Westernblot方法分析对胞内c fos蛋白的作用。亦使用流式细胞术和免疫组化方法分析PDGF受体的表达。结果 Dami细胞表达PDGF受体, 而且PDGFβ受体水平高于PDGFα受体水平。通过该受体, PDGF上调胞内c fos蛋白的表达和促进Dami细胞增殖和集落生成。结论 PDGF可能通过肿瘤细胞表面的PDGF受体, 激活胞内信息通路, 刺激细胞的增殖和集落形成。提示血小板在肿瘤的发生发展中有一定的作用Abstract: Objective It could help us to understand the role of PDGF in leukemogesis. Methods Dami cells were used as a tumor cell model to investigate the effect of PDGF on this cell proliferation and colony formation. Western blot was used to detect the effect of PDGF on c fos expression of these cells. Flow cytometry and immunocytochemical staining were also used to identify PDGF receptor in Dami cells. Results Both PDGF receptors were identified on Dami cells but not on the control cell M 07e. PDGF beta recepto...
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