肝癌细胞端粒酶逆转录酶(htert)活性与c-myc表达的关系及调控研究

Human telomerase reverse transcriptase (htert) activity correlates with c-myc expression and regulation in hepatoma cell lines

摘要: 目的　研究肝癌细胞端粒酶逆转录酶 (htert)活性与c myc的表达及其在肝癌发生中的调控机制。方法　TRAP ELISA法和RT PCR分别测定肝癌细胞株端粒酶活性、htert和c myc表达 ;将c myc表达质粒与含有htert启动子序列的报告基因质粒共转染COS 7细胞后测定启动子活性。结果　肝癌细胞株均有端粒酶活性表达 ;htert和c mycmRNA表达明显增高 ;外源性c myc可上调htert启动子活性。结论　在肝癌细胞株中, c myc和htert的表达均增强且有着重要的相关性 ;c myc对htert基因调控可能是激活端粒酶诱发肝癌发生的重要途径。

Abstract: Objective To investigate the activity of human telomerase reverse transcriptase (htert) and the expression of c-myc in hepatoma cell lines, which may regulate the pathway in hepatic tumourigenesis. Methods TRAP-ELISA method was used to determine the telomerase activity in hepatoma cell lines, and the expression of htert and c-myc in transcriptional level was measured by using RT-PCR technique. Then the c-myc expression plasmid and the vector which harboring htert promoter were co-transfected into C...