MNU诱导的大白鼠膀胱肿瘤动物模型

Expermental Model of Bladder Tumors in Rats In duced by MNU

摘要: 目的　研究N--甲基亚硝基脲(MNU)诱发大白鼠膀胱肿瘤的作用。方法　以MNU为诱癌剂，进行大白鼠膀胱灌注，以光镜、扫描电镜、透射电镜等为观察手段，对其诱发的膀胱肿瘤进行观察。结果　每次灌注MNU2 m g/只，每2周1次，共4次，8周诱发膀胱肿瘤率100%，其组织学形态及病理特征与人膀胱肿瘤十分相似。结论　MNU诱发的大白鼠膀胱肿瘤为一理想的动物模型。

Abstract: Objectivc To study the experimental animal model of b la dder tumors induced by MNU. Methods Bladder tumors were in duced in female Wistar rats by intravesicular adminstration of the carcinogen,N-methy l -N-nitrosourea(MNU).The morphological alterations bladder epithelial cells we r e stuied by light micoscopy scanning and transmission electron microscopy. Results The administration way of a total dose of 8 mg on 4 fraction o f 2 mg at 2- weekly intrvals produed a100%incidence of bladder toumor after 8 we eks.Histopathological characteristics of bladder tumor induced by MNU were b asic ally similar to those of human bladder tumor. Conclusion Intra ve sicular administration of MNU in rats provided a useful model for experiment al studies of bladder tumor.