哈萨克族食管癌差异表达基因的研究

doi:10.3971/j.issn.1000-8578.638

哈萨克族食管癌差异表达基因的研究

Screening and Cloning Differentially Expressed Genes of Esophageal Cancer in Hazak

摘要

摘要: 目的筛选并克隆在哈萨克族食管鳞癌与正常食管组织之间差异表达的基因。方法 (1)应用抑制性消减杂交(SSH)进行基因差异表达分析，构建哈族食管鳞癌组织和正常食管黏膜组织之间差异表达的cDNA消减文库；(2)克隆、鉴定食管癌组织特异表达的基因；(3)登录Genbank．运用Blastn程序进行同源性分析。结果成功构建了消减效率高的哈族食管鳞癌cDNA消成文库．对其中6个克隆的插入cDNA片断进行测序，经检索Genbank表明：这些差异表达基因与跨膜受体、乳腺癌转录因子、蛋白剪接基因及染色体1、8不同区域有较高的同源性。结论通过抑制性消减杂交技术构建了哈族食管鳞癌cDNA消减文库，并分析鉴定了部分差异表达基因，为进一步筛选鉴定食菅鳞癌特异性基因及其全长克隆、功能研究等提供了依据。

Abstract: Objective 　To clone and identify the differentially expressed genes which are related to Hazak′sesophageal cancer. Methods 　(1) Using suppression subt ractive hybridization ( SSH) to const ruct a subt ractive library containing differentially expressed genes′f ragment s ; (2) Cloning and identifying these special genes ; (3) Homology analysis of cDNA f ragment s f rom SSH by Blastn through Genbank. Results 　The differentially expressed gene cDNA library for Hazak′s Esophageal cancer was successfully const ructed . Through Blastn analysis, sequences of 6 positive clones showed that they were homologous with the genes related t ransmembrane helix receptor, breast cancer putative t ranscription factor, spliced protein gene, chromosome 8, clone RP11-27N21, clone RP11-301 G21 on chromosome 1 published in GenBank. Conclusion 　The differentially expressed gene cDNA library from Hazak′s esophageal cancer was successfully const ructed and it would lay the foundation for further screening full2length genes in Hazak′s esophageal cancer.

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