1-氧-4-羟氨基喹啉在小鼠肝肺组织中的还原性酶代谢

doi:10.3971/j.issn.1000-8578.631

1-氧-4-羟氨基喹啉在小鼠肝肺组织中的还原性酶代谢

Enzymatic Reduction of 4-Hydroxyaminoquinoline 1-oxide to 4-Aminoquinoline 1-oxide in Mouse Liver and Lung

摘要

摘要: 目的　探讨1-氧-4-羟氨基喹啉(4HAQO)还原酶在小鼠肝线粒体及微粒体和肺微粒体中的分布情况及其特征。方法　取小鼠肝肺组织碾成悬液，用离心法分离出线粒体及微粒体。当4HAQO与作为酶源的线粒体或微粒体在无氧环境和一定条件下孵化后，用分光光度法检测在反应液中还原性产物1-氧-4-氨基喹淋(4AQO)，所产生4AQO的量与孵育时间及酶源的量成直线关系。结果　肝脏中4HAQO还原酶活性是肺脏中的2.3倍，且几乎均等分布在肝线粒体及微粒体中。在反应体系中，当NADPH作为供电子体时，酶活性要比NADH作为供电子体时增高。黄素有增强4HAQO还原酶活性的作用。结论　4HAQO还原酶活性主要分布在肝线粒体及微粒体中。

Abstract: Objective The 4-hydroxyaminoquinoline 1-oxide reductase assay was optimized and validated using microsomal and mitochondrial fractions from mouse liver and microsomal fraction from mouse lung as the enzyme source. Methods Mouse liver and lung were weighted and minced,and subsequently were homogenized.Fractions of microsomes and mitochondria were isolated by centrifugation.In incudations of 4-hydroxyaminoquinoline 1-oxide with the enzyme source in anaerobic environment,the reduced product:4AQO was extracted and detected by spectrophotometric analysis.The production of 4-aminoquinoline 1-oxide was shown to be linear with incubation time for 30 minutes.Also,the production of 4-aminoquinoline 1-oxide was proportional to the amounts of microsomal and mitochodrial fractions in the incubations.The specific activity of 4-hydroxyaminoquinoline 1-oxide reductase was found to be nearly equal between microsomes and mitochondria of mouse liver. Results The spectific activity in liver microsomes was 2.3 times than in the lung microsomes.Activity observed in the presence of NADH was somewhat lower than with NADPH.Reduction of 4-hydroxyaminoquinoline 1-oxide was found to be stimulated by flavin coenzymes. Conclusion The 4-hydroxyaminoquinoline 1-oxide reductase mainly exists in microsomal and mitochondrial fractions of mouse liver.

HTML全文

参考文献(0)

施引文献

资源附件(0)