转4-1BBL 基因的小鼠肝癌细胞瘤苗刺激脾细胞产生细胞因子的研究

Cytokines Production of Murine Spleen Cells Induced by Gene Transfer of 4-1BBL into Hepatocellular Carcinoma Hepa1-6 in Vitro

摘要: 目的研究转4-1BBL基因的小鼠肝癌细胞瘤苗体外刺激同系小鼠脾细胞产生细胞因子（IL-2、TNF-α和GM-CSF）的能力。方法以丝裂霉素C（MMC）处理高表达转m4-1 BBL基因的小鼠Hepa1-6肝癌细胞，制成肿瘤细胞瘤苗（TCV），体外与同系小鼠脾淋巴细胞共同培养后，观察其对脾细胞产生细胞因子（IL-2、TNF-α和GM—CSF）的影响。结果 TCV-4-1 BBL刺激后，脾细胞体外分泌细胞因子IL-2、TNF-α和GM-CSF的水平明显增高。结论转4-1BBL基因的小鼠肝癌细胞瘤苗能刺激脾细胞产生细胞因子IL-2、TNF-α和GM-CSF。

Abstract: Objective 　To study the cytokines production of spleen cells induced in vitro by murine 4-1BBL gene transfected Hepa1-6. Methods 　Murine hepatocellular carcinoma cell line Hepa1-6-m4-1BBL which could highly express murine 4-1BBL was cultivated previously. The tumor cell vaccine ( TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC) . Cocultivation TCV with syngeneic murine spleen cells and the supernatant s were harvested for detecting the cytokines ( IL-2, TNF-α and GM-CSF) . Results 　Comparing with TCV-Hepa1-6, high levels of IL-2, TNF-α and GM-CSF were released by the spleen cells after stimulated by TCV-m4-1BBL. Conclusion 　Stimulation signals delivered by 4-BBL remarkably increased the production of IL-2, TNF-α and GM-CSF.