p53 、MDR 基因在食管癌及癌旁组织中表达的分析

doi:10.3971/j.issn.1000-8578.560

p53 、MDR 基因在食管癌及癌旁组织中表达的分析

Analysis of Expression of p53 MDR Genes in the Esophageal Squamous Cell Carcinoma and Cancerous Beside

摘要

摘要: 目的探讨食管癌和癌旁组织中p53、MDR基因的表达及其临床意义。方法采用免疫组化ABC法及逆转录酶-聚合酶链反应的方法，对50例食管癌和37例癌旁组织中p53、MDR基因的表达情况进行检测。结果 p53基因蛋白在食管癌中有显著表达为68％（34／50），癌旁组织中有表达为59％（22／37）；其中在癌旁正常组织中占54％（13／24），在癌旁非典型增生中占69％（9／13）。MDR基因在食管癌高、中、低分化中的表达水平有极显著意义，癌与正常组织有极显著意义。在不同年龄、性别、肿瘤大小上p53、MDR基因的表达无显著意义。结论 p53、MDR基因在食管癌中均呈高水平表达，癌旁组织中MDR有表达，p53基因突变早于组织形态学改变。

Abstract: Objective 　To study clinicophcthologic significance of expression of p53, MDR genes in esophogeal squamous cell carcinoma and cancerous beside. Methods 　Histochemisty ABC and RT-PCR method was used to detect the expression of p53, MDR genes in 50 carcinoma and 37 cancerous beside. Results 　p53 gene protein was marked expression in the cancer 68 %(34/ 50), and was expression in the cancerous beside 59 %(22/ 37), among 54 %(13/ 24) was normal, 69 %(9/ 13) was atypical hyperplasia ; Expression of Mdr-1 was marked difference, in turmor of high middle, low differentiation, has marked difference in cancer and normal tissue, and both no differences in ages, sex, size of tumor. Conclusion 　p53, MDR genes was high level experssion in the esophagus carcinoma MDR was experssioned in the cancerous beside, p53 gene mutation was early than change of histology forming.

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