米非司酮对子宫内膜癌细胞周期时相调控的研究
Study on the Controlling of Mifepristone on Cell Cycle of Endometrial Carcinoma
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摘要: 目的 探讨抗孕激素米非司酮对子宫内膜癌细胞周期时相的调控作用. 方法 体外培养子宫内膜癌HHUA细胞,不同浓度米非司酮处理细胞24～96小时,流式细胞术（FCM）测定癌细胞周期分布的变化;免疫组化法观察细胞周期调控蛋白的表达变化. 结果 当抗孕激素米非司酮浓度≥5μmol/L作用癌细胞36小时,G1期细胞比率明显上升,S期细胞比率（SPF）降低（P〈0.05）;细胞周期调控蛋白p21和p16表达明显上调（P〈0.05）. 结论 抗孕激素米非司酮通过调节细胞周期相关蛋白表达使HHUA细胞阻滞于G1期,抑制癌细胞增殖.Abstract: Objective To study the effect of antiprogestin mifepristone on cell cycle of endomet rial carcinoma. Methods Human endometrial carcinoma HHUA cell was cultured in vitro and t reated with mifepristone in different concent ration for 24-96 hours. Influence of mifepristone on the cell cycle dist ribution were detected by using flow cytometry ( FCM) . The expression of protein related cell cycle was observed by immunohistochemical. Results Treated HHUA cell in vit ro with mifepristone ≥5μmol/ L for 36 hours, rate of G1phase was increased and S-phase f raction (SPF) was decreased obviously ( P < 0. 05) . The expression of cell cycle related protein p21 and p16 were raised significantly ( P < 0. 05) . Conclusion Antiprogestins mifepristone could effectively block HHUA cells at G1 phase and inhibit the proliferation by cont rolling the expression of protein related cell cycle.
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