PCR-SSCP法检测非小细胞肺癌中FADD基因突变的研究
PCR-SSCP Analysis of FADD Gene Mutation in Non-small Cell Lung Cancer
-
摘要: 目的检测Fas相关死亡结构域蛋白(Fasassociateddeathdomainprotein,FADD)基因在人非小细胞肺癌(nonsmallcelllungcancer,NSCLC)中的突变情况,以探讨该基因在肺癌发生发展中的作用及机制。方法采用聚合酶链反应-单链构象多态性分析(polymerasechainreactionandsinglestrandconformationpolymorphism,PCRSSCP)检测74例NSCLC原发灶癌组织及13例癌旁正常肺组织中FADD基因突变情况。结果74例NSCLC组织中检出5例发生FADD基因突变,FADD基因突变与癌的淋巴结转移呈显著正相关(rs=0.378,P=0.001),与其他临床病理特征无关。结论NSCLC中存在着FADD基因突变。FADD基因突变在NSCLC的发生发展中可能起着重要作用。Abstract: Objective This report aimed at the mutation of FADD gene in human non-small cell lung cancer, and the evaluation of its possible effect and mechanism on the development of NSCLC. Methods PCR-SSCP was used to detect FADD gene mutation in 74 specimens from primary site of NSCLC tissues and 13 samples of normal adjacent lung tissues. Results Altogether there were 5 cases of FADD gene mutation, and the analysis revealed that its mutation was positively correlated to the status of lymph node metastasis (r_ s= 0....
下载:
