CIK细胞对乳腺癌细胞株抗增殖及诱导凋亡的作用
Anti-proliferation and Inducing Apoptosis Effects of CIK Cells Against Breast Cancer Cell Lines
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摘要: 目的研究多种细胞因子诱导的杀伤细胞(Cytokine-inducedkillcells,CIK)对ZK-75-1乳腺癌细胞株的抗增殖和诱导凋亡作用,并探讨其作用机制。方法应用MTT法检测CIK细胞对ZK-75-1乳腺癌细胞株的抗增殖作用的影响及细胞毒活性;通过倒置显微镜、HE染色、透射电镜观察凋亡细胞的形态学改变。结果MTT比色法表明随着CIK细胞与乳腺癌细胞效靶比增加及作用时间延长,抑制率明显增强(P<0.01)。CIK细胞作用于乳腺癌细胞24h后,形态学观察乳腺癌细胞发生凋亡或坏死。结论CIK细胞是一种新型高效具有较强杀伤体外乳腺癌细胞的免疫活性细胞。Abstract: Objective To study the anti-proliferation and inducing apoptosis effects of cytokine-induced killer cells-CIK cells on ZK-75-1 breast cancer cell lines and to probe its underlying mechanism. Methods To detect the anti-proliferation and the cytotoxicity of CIK cells on ZK-75-1 breast cancer line by MTT assay.The morphological changes of the apoptosis cell were observed by invert microscope,HE stain, and transmission electron microscope. Results MTT assay showed that the inhibitive rate enhanced obviously wit...
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