N糖基化抑制对脑胶质瘤细胞株SWO-38 N-GSLs合成的影响

Study on the Effects of N-glycosylation Inhibition on the Expression of N-GSLs in Glioma Cell Line SWO-38

摘要: 目的　探讨N糖基化抑制对肿瘤细胞中性鞘糖脂(N-GSLs)合成的影响。方法　以衣霉素(Tunicamycin)为N-糖基化抑制剂，观察其对胶质瘤细胞株SWO-38糖蛋白及N-GSLs合成的影响。结果　当衣霉素浓度≥5ug/ml，作用时间≥8小时，它可以在体外选择性地抑制N糖蛋白的合成，同时也抑制N-GSLs的合成，而对N-GSLs单一组分的相对百分含量无明显影响。结论　N-糖基化抑制剂在抑制细胞N糖蛋白合成的同时，对细胞N-GSLs的合成也有抑制作用。

Abstract: Objective To study the effects of N-glycosylation inhibition on the expression of neutral glycosphingolipids(N-GSLs)in cells. Methods The influence of tunicamycin on the expression of N-glycoprotein and N-GSLs in glioma cell line SWO-38 in vitro was studied. Results When incubated with tunicamycin 5ug/ml for more than eight hours,the cells' expression of N-glycoprotein and N-GSLs was inhibited. Conclusion At the time and concentration of N-glycosylation inhibitor influence on the expression of N-glycoprotein,it can also inhibit the expression of N-GSLs in cells.