冷冻联合rhTNF-α对C6大鼠脑胶质瘤细胞的凋亡诱导和增殖抑制作用

Inhibition of Proliferation and Induction of Apoptosis by Freeze Combined with rhTNF-α in Rat C6 Brain Glioma Cell

摘要: 目的探讨冷冻联合rhTNF-α对C6大鼠脑胶质瘤细胞凋亡的影响及对肿瘤生长的抑制作用。方法建立大鼠脑胶质瘤模型,待肿瘤长至第15天,随机将荷瘤鼠分为4组:G1、G2、G3、G4分别为盐水组、冷冻治疗组、rhTNF-α治疗照组及联合治疗组,同时分别行相应的治疗。于治疗后第15天取材,以TUNEL法与流式细胞仪联合检测肿瘤细胞的凋亡,采用免疫组化观察PCNA的表达,计算抑瘤率。结果联合治疗组肿瘤细胞凋亡情况与其他各组相比有显著差异(P<0.01),PCNA的表达明显减少(P<0.01),且该组对肿瘤生长的抑制率(60.38%)明显大于冷冻组(42.69%)和TNF-α组(21.68%),且大于理论抑瘤率(55.11%)。结论冷冻和rhTNF-α对C6大鼠脑胶质瘤细胞的生长均有抑制作用,同时可诱导其凋亡,两者联用抗肿瘤作用进一步加强,具有协同作用,可能成为胶质瘤治疗的一种新策略。

Abstract: Objective To investigate the effect of freeze combined with rhTNF-α on the cell apoptosis and growth of rat C6 brain glioma. Methods At 15th day after rat C6 brain glioma model being established,experiment animals were divided randomly into 4 groups: (G1)saline group; (G2)cryotherapy group; (G3) rhTNF-αgroup; (G4)combined group.And the corresponding treatment was performed according to the different groups.At 15th day following treatment,TUNEL and FCM were applied to detect apoptosis.PCNA expression was exam...