Abstract: Objective The purpose of this study is to explore the effects of silence of EDAG-1 on the growth of leukemia cell line and its mechanism. Methods HEL was infected by the virus supernatant collected from packing cell line transfected by recombinant retroviral vector, the stable cell lines were selected with puromycin. The reduction of EDAG-1 was inspected with RT-PCR; and proliferation was assayed with MTT. The tumor growth of the null mice was analyzed after injection HEL/siRNA、HEL/ negative into the skin. Results The stable HEL cell lines with a persistent knockdown of EDAG-1 were established. Comparison with HEL/ negative, the proliferation of HEL/siRNA was inhibited significantly（P<0.05）, the growth of HEL/siRNA implanted tumor slowed down significantly（P<0.05）. Conclusion Inhibition of the expression of EDAG-1 can reduce the proliferation of leukemia cell lines，suggesting that EDAG-1 may be an effective target for the treatment of leukemia.