Abstract:
Objective To investigate antitumor effects induced by dendritic cells (DCs) pulsed with complex of tumor antigen from elemene-combo tumor cell vaccine-heat shock protein 70 of BCG ( H
TA-HSP70
BCG) . Methods Tumor antigen peptides from elemene-combo Hca-F cell ( H
TA ) combined with HSP70
BCGinto H
TA-HSP70
BCG in vitro. DCs were induced in medium with GM-CSF and IL-4 and pulsed with H
TA-HSP70
BCG, H
TA and HSP70
BCG, respectively. The proliferation stimulating effects on un-separated splenocytes and the cytotoxicity of the splenocytes activated with DCs were evaluated with MTT assay. The expression of CD40 and CD86 on the surface of DCs was assessed by flow cytometer. Results Pulsing of H
TA-HSP70
BCG, resulted in DCs maturation, characterized by up-regulation of CD86 and CD40. Proliferation index of un-separated splenocytes from H
TA-HSP70
BCG group was significantly increased as compared with H
TA group . Un-separated splenocytes from DCs pulsed with H
TA-HSP70
BCG revealed the cytotoxicity agaist Hca-F, The H
TA failed to reveal the cytotoxicity against Hca-F. Conclusion H
TA-HSP70
BCG could induce DCs maturation and the mature DCs could activate splenocytes to generate more potent specific antitumor effect .
下载:
