Abstract: Objective To prepare a bispecific antibody (BsAb) against CD3 and TNP and to test its cytotoxic effect against the gastric cancer cell line,MGC803. Methods In this study,the bispecific antibody was obtained from chemical crosslinked anti-CD3 McAb with anti-TNP McAb.Using this bispecific McAb,human peripheral blood lymphocytes were induced to cytotoxic test of MGC803 tumour cells directed by different TNP-McAb. Results BsAb combined with TNP-McAb mediated cytotoxicity against MGC803 was much more effective than that combined with no TNP-McAb.The cell lytic effect was proportional to the ratio of effecter cells to target cells. Conclusion Anti-CD3／Anti-TNP with versatile adaptor could mediate different antibody that crosslinked with TNP,and has potential value in clinic.