肿瘤患者T细胞克隆性增殖与预后的分析
Analysis of T Cell Clonal Proliferation and the Prognosis in Patients with Tumors
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摘要: 目的 通过分析肿瘤患者肿瘤浸润性淋巴细胞 (tumor infiltratinglymphocyte, TIL)和外周血淋巴细胞 ( peripheralbloodlymphocytes,PBL)中克隆性增殖T细胞的TCRβ基因谱型的分子特征, 研究T细胞免疫在肿瘤预后中的作用。方法 采用RT PCR及变性聚丙烯酰胺测序凝胶电泳方法扩增并分离TCRβ基因V J重排片断。通过PCR产物直接测序的方法, 判断T细胞克隆的特征, 得到CDR3区氨基酸序列。结果 在结直肠癌、肺癌TIL和术前PBL中均发现TCRβ基因的寡克隆性扩增, 它们在术后的PBL中均消失。具有术前PBL的克隆性增殖T细胞的患者还可能影响肿瘤的预后。结论 肿瘤患者的肿瘤组织和术前外周血中都存在肿瘤肽特异性激活的T细胞克隆, T细胞免疫系统的激活在肿瘤的预后中可能起着重要作用Abstract: Objective To study T cell immunology in patients with solid cancers, the molecular features of clonal expanded T cell in TIL and PBL were analyzed by assessment of TCRβ gene repertoire. Methods The rearrangement of V J genes in TCRβ chain was amplified by the method of RT PCR with denaturation polyacrylamide sequencing gel eletrophoresis. The PCR production was sequenced directly to determine the characteristics of the clonal expanded transcripts. Results There were oligoclonal expanded transcripts of TC...
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