胸苷酸合成酶对结肠癌5-Fu化疗耐药影响的前瞻性研究

The Influence of Thymidylate Synthase (TS) in Colon Carcinoma used 5-Fu Cure Prospective Study

摘要: 目的　探讨结肠癌对 5 Fu产生耐药的机理, 从细胞培养水平上进一步论证胸苷酸合成酶对 5 Fu化疗产生耐药的影响。方法　应用MTT法对体外培养的 30例结肠癌细胞进行 5 Fu药敏试验, 再应用SP免疫组化法对 30例结肠癌进行胸苷酸合成酶检测, 然后将两个结果进行比较。结果　胸苷酸合成酶高表达组体外培养的结肠癌细胞对 5 Fu的敏感性明显低于TS低表达组。结论　胸苷酸合成酶表达程度的高低对临床结肠癌患者化疗药物的选择有一定的指导意义

Abstract: Objective To investigate the reason of the 5 Fu drug resistance, and to prove deeply the influence of TS in colon carcinoma with the level of cell culture. Methods To proceed the 5 Fu drug susceptibility test with MTT method to 30 cases in vitro culture colon carcinoma cell; to be examined by S P immunohistochemical staining and observed the expression of TS; and then compare with them. Results In vitro culture colon carcinoma cells corresponding high expression of TS the senility to 5 Fu delay obviou...