流式细胞术测定COX-2、iNOS基因蛋白在食管癌上的表达及意义

Expression and Significance of COX-2 and iNOS in Esophageal Cancer by Flow Cytometry

摘要: 目的　探讨环氧化酶 2 (COX 2 ), Ⅱ型一氧化氮合酶 (iNOS)基因蛋白在食管癌上的表达以及与食管癌的分化和淋巴结转移的关系。方法　采用流式细胞术定量检测 6 5例食管鳞状细胞癌上COX 2、iNOS的蛋白表达量 (用荧光指数FI表示 )。结果　COX 2、iNOS在低分化型 (G3)鳞癌上的表达量明显高于分化型 (G1、G2 )鳞癌 (其P值分别为 0 .0 0 0 1、0 .0 385 ) ;二者之间呈明显正相关。这两种基因蛋白的表达强弱均与淋巴结转移无关。结论　COX 2、iNOS的表达强弱与食管癌的分化密切相关 ;COX 2与i NOS在促食管癌的发生发展中有互相协同作用

Abstract: Objective To study the expressions and significance of COX 2,iNOS proteins in esophageal squamous cell cancer(ESCC) and their relationship with tumor differentiation,lymph nodes metastasis. Methods The expressions of COX 2,iNOS proteins in 65 cases of ESCC were detected quantitatively by Flow Cytometry. Results The expressions of COX 2,iNOS in poorly differentiated ESCC were higher than those of well differentiated ESCC( P = 0.0001, 0.0385 ). There was positive correlation between COX 2 and iN...