喉癌细胞PCNA表达和DNA含量与临床预后的关系

doi:10.3971/j.issn.1000-8578.317

喉癌细胞PCNA表达和DNA含量与临床预后的关系

Prognostic Implications of PCNA Expression and DNA Contents in Squamous Cell Carcinoma of Larynx

摘要

摘要: 目的　探讨增殖细胞核抗原(PCNA)的表达和DNA含量对喉癌临床生物学特征及预后的关系。方法　用免疫组化SP方法和自动图像分析仪检测正常喉粘膜、喉鳞癌的PCNA和DNA指数(DI)，结合临床随访资料进行分析。结果　喉鳞癌PCNA的阳性表达率为100%，与正常喉组织有显著差异(P<0.01)，PCNA阳性表达强度和DNA指数随肿瘤分化程度的降低而相应增高(P<0.05)。两指标较高者生存时间较短，预后较差(P<0.05)。结论　PCNA的表达和DNA指数可为判断肿瘤的恶性程度及临床预后提供参考依据。

Abstract: Objective To investigate Proliferating cell nuclear antigen(PCNA) and DNA index(DI) correlation to biological characteristics of laryngeal carcinoma and their values of clinical prognosis. Methods Immunohistochemical staining was used to detect the expression of PCNA in laryngeal cancer and normal tissue,and with MIPS-I image analysis system DNA contents of cancer cell were measured and made out DNA index. Results PCNA positive rate was 100% in laryngeal carcinoma,and it was negative in all 10 normal laryngeal mucosa specimens(P<0.01).The positive expression of PCNA and DNA contents in the laryngeal carcinoma were increased with the differentiation of tumors lower(P<0.05).With the increasing PCNA positive expression and DI,the prognosis of the patients got poorer(P<0.05). Conclusion PCNA and DI were simultaneously detected can be used as the prediction of tumor malignancy and prognosis.

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