肝外胆管癌中染色体9p21区段抑癌基因簇表达异常的研究

The Study of Relationship between Inactivation of Tumor Suppressor Gene Cluster Located in Chromosome 9p21 and Progression of Extra-hepatic Bile Duct Cancer

摘要: 目的　研究位于 9号染色体短臂 2 1段的抑癌基因簇所包含的 3个抑癌基因 p14 /ARF、p15 /INK4B和p16 /INK4A在肝外胆管癌组织中的异常表达, 探讨这 3个抑癌基因的表达异常在肝外胆管癌的发生发展过程中的作用。方法　应用免疫组织化学方法研究肝外胆管癌肿瘤组织、癌旁组织以及胆管炎组织的石蜡切片中p14、p15和 p16蛋白的表达。结果　肝外胆管癌组织、癌旁组织以及胆管炎组织中 p14 /ARF、p16 /INK4A两个基因表达缺失率两两比较均有显著性差异 (P <0 .0 5 ), 而 p15 /INK4B基因在各组中的表达无显著性差异 (P >0 .0 5 )。 3个基因的表达异常与肿瘤的组织学类型无关, 在伴有淋巴结转移 /局部浸润的病例中缺失率明显增高 (P <0 .0 5 )。结论　p14 /ARF、p16 /INK4A基因表达异常早期参与了肝外胆管癌发生、发展

Abstract: Objective To study the abnormal expression of three tumor suppressor p14/ARF, p15/INK4B and p16/INK4A located in chromosome 9p21 in the tissue of extra-hepatic bile duct cancer, and to discuss the possible role of these three genes in the initiation and progression of bile duct cancer. Methods The expression of p14, p15 and p16 proteins in the tissues of tumor, tumor side and cholangitis were studied using the method of immunohistochemistry. Results The rates of p14/ARF and p16/INK4A gene expression loss ...