人突变p27基因对大肠癌细胞凋亡的影响

Effects of Mutant p27 Gene on the Apoptosis of Colorectal Cancer Cell

摘要: 目的探讨人突变p27基因对大肠癌细胞凋亡的诱导作用。方法构建重组腺病毒Ad-p27mt,转染大肠癌细胞SW480,用流式细胞仪、DNA片段分析法、TUNEL法观察Ad-p27mt对大肠癌细胞的凋亡的诱导作用。结果成功构建了Ad-p27mt,在感染复数≥50时,可达100%的转导效率。Ad-p27mt转染SW480。24h后流式细胞仪检测在G1期前出现了亚二倍体凋亡峰,细胞DNA提取电泳后出现了凋亡特征性梯带,TUNEL法检测凋亡指数实验组和对照组分别为(82.0±3.2)和(5.0±3.5),差异有显著性(P<0.01)。结论人突变p27基因能有效诱导大肠癌细胞的凋亡。

Abstract: Objective To study the inducing effect of human mutant p27 gene on apoptosis of the colorectal cancer cells. Methods The recombinant Adenovirus Ad-p27mt was constructed to infect the colorectal cancer cell SW480.The inducing effect of Ad-p27 mt on apoptosis of the colorectal cancer was measured by the flow cytometer,the DNA fragment analysis and TUNEL method. Results Ad-p27mt was successfully constructed.If the multiplicity of infection (MOI) was ≥50,the infection efficiency can reach 100%.After 24h of infect...