Abstract: Objective 　To investigate the effect s of knockdown of c2Met by adenovirus2delivered siRNA on the growth of hepatocellular carcinoma cells in vit ro. Methods 　c2Met was knocked down by adenovirus2 delivered siRNA. The expression of protein was detected by Western blot . Cell proliferation was deter2 mined by MTT assay and Cell count experiment s. Cell cycle was analyzed by flow cytomet ry. Cell colony formation ability was assessed by sof t agar colony formation assay. Results 　The adenovirus2delivered siRNA could decrease the c2Met expression of HCCLM3 cells by over 90 %. Knockdown of c2Met could arrest HCCLM3 cells at G1 / G0 and inhibit the proliferation of HCCLM3 cells by over 30 %( P < 0. 01) . Furthermore, Knockdown of c2Met could reduce HCCLM3 cells colony formation by 78 % ( P < 0. 01) . Conclusion 　Knockdown of c2Met expression by adenovirus2delivered siRNA may be a potential approach for hepatocellular carcinoma gene therapy.