葛根黄酮提取物对HL-60细胞增殖和凋亡的影响
Effect of Kudzu Flavonoids Extracts on Proliferation and Apoptosis of HL-60 Cells
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摘要: 目的 观察葛根黄酮提取物对HL-60细胞的影响及作用机制。方法 用MTT测定对HL-60细胞增殖的影响;用流式细胞仪检测细胞周期变化及凋亡情况;采用NBT比色法检测诱导HL-60细胞分化的能力;DNALadder检测HL-60细胞的凋亡特征及效果;用TUNEL检测体内诱导凋亡能力。结果 葛根黄酮提取物有较强抑制HL-60细胞生长和增殖能力,处理3d可出现明显的凋亡特征,凋亡率可达38.3%,其诱导分化能力弱,葛根黄酮提取物的主要成分葛根素和大豆甙元的诱导凋亡能力弱,但大豆甙元具有较强的诱导HL-60细胞分化的能力,葛根黄酮提取物有体内诱导凋亡抗肿瘤能力。结论 葛根黄酮提取物可通过诱导细胞凋亡抗人白血病HL-60细胞的生长。Abstract: Objective To investigate the effect of Kudzu flavonoids extracts on human leukaemia cancer cells HL-60 line to reveal its probable mechanism. Methods The survival and proliferation of HL-60 cells were determined by MTT.The cell-DNA ploidy distribution and apoptotic rate were measured by flow cytometry (FCM), and DNA ladder.The differentiation induced potence was determined by NBT. The anticancer and apoptosis potence induced by flavonoids detected by TUNEL. Results There was significant inhibition of the ce...
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