c-myc反义核酸对乳腺癌MCF-7 细胞生长及hTERT基因表达的影响

doi:10.3971/j.issn.1000-8578.2422

c-myc反义核酸对乳腺癌MCF-7 细胞生长及hTERT基因表达的影响

Effect of c-myc Antisense Oligodeoxynucleotides on Cytology and Human Telomerase Reverse Transcriptase Gene in MCF-7 Cells

摘要

摘要: 目的探讨脂质体介导c-myc基因反义寡核苷酸（Antisense phosphorothioate oligodeoxynueleotide，ASODN）对MCF-7细胞生长及其人体端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）基因表达的影响。方法将c-myc正、反义寡核苷酸（ODN）分别导入各组MCF-7细胞中，MTT法、逆转录-聚合酶链法及流式细胞术分别检测各组细胞生长情况、hTERT mRNA的表达水平以及细胞凋亡率。结果 c-myc ASODN转染MCF-7细胞24h后，细胞生长受到抑制（P〈0．05），并且hTERT mRNA表达明显降低；随着反义核酸作用时闰的延长，凋亡细胞数目逐渐增多，细胞生长及hTERT表达的下降随时阃延长逐渐明显。对照组、LR-SODN组、LR-ASODN 24h组与LR-ASODN 48h、72h组相比有明显统计学差异（P〈0．05）。结论 c-myc反义寡核苷酸能显著下调细胞中hTERT基因的表达活性，诱导MCF-7细胞凋亡，并抑制MCF-7细胞生长；在一定程度上，其效果与时间呈正相关。

Abstract: Objective 　To investigate the effect s of c-myc ASODN on MCF-7 cells growth and the expression of hTERT gene in MCF-7 cells. Methods 　After transfecting c-myc SODN and ASODN to MCF-7 cells, we measured the proliferation, apoptosis rates and the expression of hTERT mRNA respectively by MTT method, flow cytomet ry analysis and RT-PCR. Results 　When c-myc ASODN affected MCR-7 cells at 24h, cells growth was inhibited significantly ( P < 0. 05) with hTERT gene expression of MCF-7 cells descending significantly ( P < 0. 01) . The apoptosis rates of the ASODN2t reated cells rised significantly at 48h. The described effect s of suppression on cells rised with time increasing. Conclusion 　c-myc ASODN can effectively inhibit the MCF-7 cells growth, induce apoptosis, and down-regulate hTERT gene expression in MCF27 cell. The effects correlated with time to a degree.

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