Abstract: Objective 　To evaluate the detected difference of tumor markers between protein biochip and ELISA method. Methods 　The serum levels of 3 common used tumor markers, including AFP, CA199, and CEA, were measured with the C-12 protein biochip detective system in 50 primary hepatic cancer patients, 17 patients with liver cirrhosis, 16 patients with chronic hepatitis and 40 healthy persons. Meanwhile, the 3 tumor markers serum levels were also detected by EL ISA. Results 　Combined measured positive rate and specificity for PHC were 78. 00 % and 82. 19 % when the 3 tumor markers were measured with protein biochip, but the positive rate and specificity were 78. 00 % and 75. 34 % by ELISA. There wasn't significant difference between these two methods ( P > 0. 05) . The coincident rate was 92. 95 %, and spearman rank correlation was 0. 842 ( P < 0. 001) . Conclusion 　Protein biochip could be measured the serum tumor markers levels accurately, and it was more quickly and conveniently than t raditional methods. It could be used as a common means for the measurement of tumor markers.