水杨酸钠诱导HL-60细胞凋亡及其与胞内游离Ca~(2+)浓度的关系

Sodium salicylate induced apoptosis in HL-60 cells and its relation to the changes of intracellular free Ca~(2+)

摘要: 目的　目的研究水杨酸钠对HL 6 0细胞的促凋亡活性及其与胞内游离Ca2 + 浓度的关系。方法Annexin V结合分析检测靶细胞磷脂酰丝氨酸外化 ;琼脂糖凝胶电泳检测断裂DNA ;激光共聚焦显微镜分析细胞内游离Ca2 + 浓度变化。结果　HL 6 0细胞经 5mmol/L(IC50 )水杨酸钠分别作用 10h、16h后, 靶细胞的磷脂酰丝氨酸外化率显著高于对照组 (0 .2 4vs 0 .0 3), 其基因组DNA在琼脂糖凝胶电泳中呈典型的梯状带型 ;此间, HL 6 0细胞内游离Ca2 + 浓度进行性升高。结论　水杨酸钠触发的HL 6 0细胞凋亡与胞内游离Ca2 + 浓度升高密切相关。

Abstract: Objective To study the proapoptotic effect of Sodium Salicylate (Na-Sal) on the HL-60 Cells and its relation ship to the changes of intracellular free Ca 2+. Methods The phosphatidylserine exposure was measured by Annexin-V binding assay; DNA fragmentation detected by agarose gel electrophoresis; and the changes of the concentration of intracellular free Ca 2+ analyzed by confocal laser scanning microscope (CLSM). Results After treated with 5mmol/L(IC 50) Na-Sal for 10hs and 16hs, respectively,...