联合检测血浆中GSH-PX、MDA 及肿瘤蛋白对肺癌筛检的Bayes 判别分析

doi:10.3971/j.issn.1000-8578.2234

联合检测血浆中GSH-PX、MDA 及肿瘤蛋白对肺癌筛检的Bayes 判别分析

Bayes Model of GSH-PX,MDA and Oncogene Proteins and Its Possible Role in The Screening of Lung Cancer

摘要

摘要: 目的探讨血浆中GSH—PX、MDA、p21、p53及HSP70蛋白实测值建立的Bayes模型对肺癌的筛检价值。方法用DTNB法对48例肺癌、34例非肿瘤性肺病病例（简称对照组）血浆中GSH-PX活力进行测定；用TAB法测定其MDA的含量；采用Western斑点印迹法测定血浆中p21、p53及HSPT0蛋白水平，并用其实测值建立的Bayes模型。结果肺癌组血浆中GSH—PX活力小于对照组，MDA高于对照组，具有显著性意3L（P〈0．05）；与对照组相比，肺癌组p21、p53及HSP70蛋白水平均增高，差异具有显著性意义（P〈0．01）；Bayes模型的敏感性、特异性和准确率分别为83．33％，74．19％和93．33％。联合细胞学检测，其诊断的敏感性可提高至91．67％。结论 GSH—PX、MDA、p21、p53及HSP70蛋白联合检测建立的Bayes模型对肺癌筛检提供了一条新的方法，可补充细胞学的诊断价值。

Abstract: Objective 　To explore the screening of lung cancer with Bayes model of GSH-PX, MDA and oncogene proteins. Methods 　plasma lipid peroxidation from 48 cases of lung cancer (LC), 34 cases of non lung cancer of respiratory diseases (cont rol group) were measured with glutathione peroxidase ( GSH-PX) and malonydialdehyde (MDA) . Western Dot Blotting was used to explore the expression of ras, p53 and heat st ress protein 70 (hsp70) in LC and cont rols. Results 　The GSH-PX activity of LC was lower than that of cont rols, P < 0. 01. The level of MDA increased more significantly in LC group than in control group, P < 0. 05. The level of p21, p53, and HSP70 in LC group were higher than those in control group, P < 0. 01. The sensitivity, specificity and the predicative rate of Bayes model were 83. 33 %, 74. 19 % and 93. 33 % respectively. Co2detection of the model and conventional cytology produced a sensitivity of 91. 67 %. Conclusion 　Bayes model of GSH-PX, MDA, p21, p53 and HSP70 might be used as the screening of lung cancer and added to the diagnostic value of conventional cytology.

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