p53p21双基因转染对肝癌细胞生长的抑制作用研究

Inhibition effect of co-expression of human wild type p53 and p21 genes on hepatocellular carcinoma

摘要: 目的　研究 p5 3和 p2 1双基因对肝癌细胞的联合基因治疗效果。方法　通过磷酸钙 -DNA共沉淀法将p5 3和 p2 1双基因真核共表达载体及单基因表达载体引入肝癌细胞SMMC 772 1,用直接镜检, DNAladder检测法和四甲基偶氮唑 (MTT)法等研究细胞生长情况。结果　转染外源 p5 3p2 1双基因的肝癌细胞SMMC 772 1与未转染及单基因转染的肝癌细胞SMMC 772 1相比, 其增殖速度显著下降。结论　p5 3和p2 1双基因对肝癌细胞的联合基因治疗效果比较明显, 对肝癌基因治疗的进一步研究具有指导意义。

Abstract: Objective Research the effect of co-gene therapy of human wild type p53 and p21 genes on hepatocellular carcinoma. Methods Construct the eucaryon vector co-expressing human wild type p53 and p21 genes. The vector with wild type p53 and p21 genes was introduced into cancer cells through methods of calcium phosphate-DNA co-deposition. Direct microscopy, DNA ladder and monotetrazolium(MTT) test was performed to measure the effect. Results It was showed that most cells were transfected and the growth rate wa...