重组人肿瘤坏死因子α衍生物治疗鼠脑胶质瘤
Derivative of recombinant human tumor necrosis factor-α therapy for rat glioma
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摘要: 目的 探讨重组人肿瘤坏死因子α衍生物 (rhTNF α)治疗鼠脑胶质瘤的有效性。方法 通过改造人肿瘤坏死因子α形成rhTNF α, 体外培养鼠脑胶质瘤细胞, 加入不同浓度rhTNF α,MTT法测定细胞存活率, 颅内立体定向种植C6细胞制成鼠脑胶质瘤模型, 10天后MRI增强测量肿瘤大小, 腹腔注射rhTNF α, 治疗后第 8天、15天、2 1天分别用MRI增强测量肿瘤大小。结果 体外实验 :加入rhTNF α 5天后, 大部分C6细胞死亡, 细胞存活率随rhTNF α的递增而降低。体内实验, 治疗 8天后肿瘤即开始缩小, 治疗 2 1天后, MRI未见明显肿瘤强化灶。结论 rhTNF α能有效治疗鼠脑胶质瘤。Abstract: Objective To investigate the effects of derivative of recombinant human tumor necrosis factor-α(rhTNF-α) therapy for rat glioma. Methods rhTNF-α were created by modifying TNF-α, were cultured in culture medium conclude rhTNF-α in different concertration. The livability of cells was determined by MTT technique five days later and implanted C 6 cells in rat s brain to establish glioma model. rhTNF-α were intraperitoneally injected 10 days after implanted. The volume of glioma were measured by MRI 10 days a...
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