食管鳞状细胞癌患者与健康人群肠道菌群的差异研究

曹燕珍; 图荪阿依·吾麦尔; 胡佳捷; 杨丽丽; 房新志

doi:10.3971/j.issn.1000-8578.2023.23.0297

食管鳞状细胞癌患者与健康人群肠道菌群的差异研究

Difference in Intestinal Flora Among Patients with Esophageal Squamous Cell Carcinoma and Normal Population

摘要

摘要:
目的探讨食管鳞状细胞癌患者与健康人群肠道菌群的差异，为肠道菌群作为食管鳞状细胞癌早期诊断标志物提供依据。
方法利用微生物扩增子测序法对30例已确诊食管鳞状细胞癌患者的活检组织样本（观察组）及同期收集的25例健康体检者食管活检组织样本（对照组）提取组织DNA，检测DNA完整性与DNA质量，对两组样本的肠道菌群组成及丰度进行测定，并分析其组间的统计学差异。
结果两组食管黏膜组织样本菌群Beta多样性方面有较大相似性，但存在一定差异，观察组患者变形菌门Proteobacteria、疣微菌门Verrucomicrobia的相对丰富度高于对照组（P<0.05）；观察组患者巨单胞菌Megamonas属的相对丰度低于对照组（P=0.025）。
结论加强对食管鳞状细胞癌患者肠道菌群变化研究可能对其防治具有重要意义。

Abstract:
Objective To investigate the difference in intestinal flora among patients with esophageal squamous cell carcinoma and normal population and to provide a basis for the early diagnosis of esophageal squamous cell carcinoma as a marker.
Methods DNA was extracted from biopsy tissue samples of 30 patients with esophageal squamous cell carcinoma (observation group) and 25 healthy people (control group) by microbial amplification sequencing. The integrity and quality of DNA were detected. The composition and abundance of intestinal flora in the samples of the two groups were determined.
Results A great similarity in beta diversity was found between the two groups, but some differences were also observed. The relative abundance of Proteobacteria and Verrucomicrobia in the observation group was higher than that in the control group (P<0.05). The relative abundance of Megamonas in the observation group was lower than that in the control group (P=0.025).
Conclusion Strengthening the study on the changes in intestinal flora among patients with esophageal squamous cell carcinoma may be of great significance for its prevention and treatment.

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