小分子抑制剂CIL56介导食管鳞状细胞癌细胞死亡

陈嘉兴; 许霞青; 张旗

doi:10.3971/j.issn.1000-8578.2023.22.1447

小分子抑制剂CIL56介导食管鳞状细胞癌细胞死亡

Effect of Small-molecule Inhibitor CIL56 on Death of Esophageal Squamous Cell Carcinoma Cells

摘要

摘要:
目的探讨小分子抑制剂CIL56介导食管鳞状细胞癌细胞死亡的作用及机制。
方法 SRB法和平板克隆法检测CIL56对食管鳞状细胞癌增殖的影响；划痕愈合实验检测CIL56对细胞迁移的影响；铁离子检测试剂盒检测CIL56对细胞铁离子浓度的影响；总谷胱甘肽试剂盒检测CIL56对细胞总谷胱甘肽浓度的影响；Western blot检测铁死亡相关蛋白xCT、GPX4和YAP1蛋白的表达。
结果小分子抑制剂CIL56能够显著抑制细胞的增殖（P < 0.05）和迁移（P < 0.001）；随着CIL56浓度的增加，食管鳞状细胞癌中铁离子的浓度随之增加（P < 0.05）；CIL56能够降低食管鳞状细胞癌中总谷胱甘肽的含量（P < 0.01）和铁死亡相关蛋白xCT和GPX4蛋白的表达（均P < 0.001），且抑制YAP1蛋白的表达。
结论小分子抑制剂CIL56能够显著抑制食管鳞状细胞癌的细胞增殖和迁移，并降低细胞中铁死亡相关蛋白xCT和GPX4蛋白以及YAP1蛋白的表达。

Abstract:
Objective To investigate the role and mechanism of the small-molecule inhibitor CIL56 in the death of esophageal squamous cell carcinoma cells.
Methods SRB method and plate-cloning method were used to detect the effect of CIL56 on the proliferation of esophageal squamous cell carcinoma. The effect of CIL56 on the migration of esophageal squamous cell carcinoma cells was investigated by scratch-healing test. The effect of CIL56 on the concentration of iron ions in esophageal squamous cell carcinoma was detected with an iron-detection kit. A total glutathione test kit was used to examine the effect of CIL56 on glutathione concentration in esophageal squamous cell carcinoma. Western blot was used to investigate the effect of CIL56 on the expression of xCT and GPX4 proteins related to iron death, as well as YAP1 protein, in esophageal squamous cell carcinoma.
Results CIL56 could significantly inhibit the proliferation (P < 0.05) and migration (P < 0.001) of esophageal squamous cell carcinoma. With the increased CIL56 concentration, the iron concentration in esophageal squamous cell carcinoma increased (P < 0.05). CIL56 could reduce the glutathione content in esophageal squamous cell carcinoma (P < 0.01). CIL56 could reduce the expression of xCT and GPX4 proteins related to iron death and decrease the level of YAP1 protein in esophageal squamous cell carcinoma (both P < 0.001).
Conclusion The small-molecule inhibitor CIL56 can significantly inhibit the proliferation and migration of esophageal squamous cell carcinoma cells and reduce the expression of the iron-death-related proteins xCT and GPX4, as well as YAP1 protein.

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