非小细胞肺癌组织中circ_0006692的表达及其对肺癌细胞增殖转移的调控机制

陈增; 廖锦容; 邹长棪; 苏颖; 林可焴; 金善丰; 郑倩兰; 林贤东

doi:10.3971/j.issn.1000-8578.2021.21.0383

非小细胞肺癌组织中circ_0006692的表达及其对肺癌细胞增殖转移的调控机制

Expression of circ_0006692 in Non-small Cell Lung Cancer and Its Regulatory Mechanism on Proliferation and Metastasis of Lung Cancer Cells

摘要

摘要:
目的探讨非小细胞肺癌(NSCLC)中circ_0006692的表达与临床病理特征的关系及机制。
方法收集50对NSCLC和癌旁组织，qRT-PCR法检测癌和癌旁组织中circ_0006692的表达，并分析其与临床病理特征之间的关系。构建过表达、敲低的circ_0006692的A549肺癌细胞株，MTS、克隆形成实验、划痕创伤愈合实验和Transwell侵袭实验检测circ_0006692表达变化对细胞增殖、迁移和侵袭能力的影响。qRT-PCR和Western blot检测circ_0006692表达变化对EMT相关基因表达的影响。
结果非小细胞肺癌组织中circ_0006692表达水平高于癌旁组织(P < 0.05)，其表达水平与肿瘤大小、TNM分期及肺膜侵犯密切相关(P < 0.05)。circ_0006692敲低抑制细胞增殖、侵袭和转移，过表达可促进细胞增殖、侵袭和转移。qRT-PCR和Western blot结果显示circ_0006692敲低抑制A549肺癌细胞EMT相关基因CDH2和MMP7促进CDH1表达。
结论非小细胞肺癌组织中circ_0006692的表达上调与肿瘤大小、TNM分期及肺膜侵犯密切相关; circ_0006692表达可调节A549肺癌细胞增殖、侵袭、转移以及EMT进展。

Abstract:
Objective To explore the expression of circ_0006692 in NSCLC and its relation with clinicopathological characteristics and related mechanism.
Methods We collected 50 pairs of NSCLC tissues and adjacent tissues. The expression of circ_0006692 was detected by qRT-PCR and its relation with clinicopathological features was analyzed. We constructed lung cancer A549 cell line with circ_0006692 overexpression and knockdown. Cell proliferation, migration and invasion were detected by MTS, colony forming, wound healing and Transwell invasion assays. qRT-PCR and Western blot were used to detect the effect of circ_0006692 expression change on EMT-related gene expression.
Results The expression of circ_0006692 in NSCLC was significantly higher than that in adjacent tissues (P < 0.05). The expression level of circ_0006692 was closely related to tumor size, TNM stage and pulmonary membrane invasion (P < 0.05). circ_0006692 knockdown inhibited cell proliferation, invasion and metastasis, while its overexpression promoted cell proliferation, invasion and metastasis. circ_0006692 knockdown inhibited the expression of EMT-related genes CDH2 and MMP7 in A549 cells and promoted the expression of CDH1.
Conclusion The upregulated expression of circ_0006692 in NSCLC is closely related to tumor size, TNM stage and pulmonary membrane invasion. circ_0006692 expression could regulate the proliferation, invasion, metastasis and EMT progression of lung cancer A549 cells.

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