Panx1调控ATP/IP3通路促进顺铂诱导A549细胞的凋亡

龙文清; 张晖力; 谢海娟; 王毓兴; 张丽君; 俞红女; 王林

doi:10.3971/j.issn.1000-8578.2021.20.1546

Panx1调控ATP/IP3通路促进顺铂诱导A549细胞的凋亡

Panx1 Promotes Cisplatin-induced Apoptosis of A549 Cells by Regulating ATP/IP3 Pathway

摘要

摘要:
目的观察顺铂诱导肺腺癌细胞凋亡过程中Panx1对ATP/IP3信号通路的调控及作用机制。
方法以人肺腺癌A549细胞为研究对象，以甘珀酸（CBX）为药物干扰工具。将人肺腺癌A549细胞分为：空白组（Control组）、甘珀酸组（CBX组）、顺铂组（DDP组）、甘珀酸+顺铂组（CBX+DDP组）。MTT法和Annexin V/PI法分别检测A549细胞存活率和凋亡率的变化。化学发光法和ELISA法分别检测细胞外三磷酸腺苷（ATP）和细胞内三磷酸肌醇（IP3）的相对浓度。
结果与单用DDP组相比，CBX+DDP组的细胞存活率增加（P < 0.01）、细胞早期凋亡率和晚期凋亡率下降（P < 0.01）、细胞外ATP、细胞内IP3的释放浓度降低（P < 0.01）。
结论 Panx1可通过调控ATP/IP3信号通路增加肺腺癌细胞对顺铂的敏感度。

Abstract:
Objective To observe the regulation of Panx1 on ATP/IP3 signaling pathway and its mechanism during cisplatin-induced apoptosis of lung adenocarcinoma cells.
Methods Human lung adenocarcinoma cell line A549 was used as the research object and carbenoxolone (CBX) was used as a drug interference tool. A549 cells were divided into normal control group, the CBX group, the cisplatin (DDP) group and the CBX+DDP group. MTT assay and Annexin V/PI assay were used to detect the survival and apoptosis rates of A549 cells. The relative concentrations of extracellular adenosine triphosphate (ATP) and intracellular inositol triphosphate (IP3) were measured by Chemiluminescence and ELISA.
Results Compared with DDP group, the cell survival rate of CBX+DDP group increased, while the early and late apoptotic rates and the release concentration of extracellular ATP and intracellular IP3 decreased (all P < 0.01).
Conclusion Panx1 can increase the sensitivity of lung adenocarcinoma cells to cisplatin by regulating the ATP/IP3 signaling pathway.

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