膀胱癌诊断与预后相关基因TPM1和CALD1的筛选及验证

赵阳; 常富江; 葛雷; 张楠; 单中杰

doi:10.3971/j.issn.1000-8578.2021.20.1536

膀胱癌诊断与预后相关基因TPM1和CALD1的筛选及验证

Screening and Verification of TPM1 and CALD1 Related to Diagnosis and Prognosis of Bladder Cancer

摘要

摘要:
目的筛选及验证膀胱癌诊断与预后相关基因TPM1和CALD1。
方法利用TCGA和GEO数据库中的数据集分别得到414例和188例膀胱癌基因芯片表达数据，通过构建加权基因共表达网络(WGCNA)及识别肿瘤组织与正常组织间差异表达基因的方法，得到与膀胱癌高度相关的枢纽基因。使用STRING数据库构建蛋白互作网络，筛选出预后相关的枢纽基因。将郑州人民医院29例膀胱癌样本及HPA数据库中的免疫组织化学结果作为外部验证结果。
结果 TCGA数据库中共筛选出915个差异表达基因，GSE13507中筛选出464个差异表达基因。通过WGCNA得到两个相关性最强的模块：蓝色模块(相关系数为0.60，P=1E-44)和青色模块(相关系数为0.52，P=7E-19)，得到156个交集基因。通过蛋白质互作网络分析筛选出10个枢纽基因，其中TPM1和CALD1基因与膀胱癌生存的相关性最大，并得到了外部验证组验证。
结论 TPM1和CALD1基因与膀胱癌预后紧密相关，也是促进肿瘤进展的枢纽基因。

Abstract:
Objective To screen and verify hub genes TPM1 and CALD1 that can affect the diagnosis and prognosis of bladder cancer (BLCA).
Methods We obtained gene chip expression data of 414 and 188 cases of bladder cancer from TCGA and GEO, respectively. By constructing a weighted gene co-expression network analysis (WGCNA) and identifying differentially-expressed genes between tumor tissues and normal tissues, the pivotal genes that were highly associated with bladder cancer were obtained, and the STRING database was used to construct a protein interaction network to screen out prognostic-related pivotal genes. We took 29 cases of bladder cancer samples from People's Hospital of Zhengzhou as external verification results.
Results A total of 915 and 464 differentially-expressed genes were screened from the TCGA database and GSE13507, respectively. Two modules with the strongest correlation were obtained through WGCNA: the blue module (Pearson cor=0.60, P=1E-44) and the cyan module (Pearson cor=0.52, P=7E-19), and 156 intersection genes were obtained. Through protein interaction network analysis, 10 pivotal genes were screened out. TPM1 and CALD1 genes had the greatest correlation with the survival of bladder cancer patients, which was verified by external experimental verification group.
Conclusion TPM1 and CALD1 are closely related to the prognosis of bladder cancer patients. They are also pivotal genes to promote tumor progression.

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