非小细胞肺癌患者外周血和肺癌组织中ZIC1启动子甲基化检测的临床意义

徐瑶; 刘世国; 张畅; 张黎; 姜姗; 张军霞; 彭黎

doi:10.3971/j.issn.1000-8578.2021.20.0911

非小细胞肺癌患者外周血和肺癌组织中ZIC1启动子甲基化检测的临床意义

Clinical Significance of Detection of ZIC1 Promoter Methylation in Peripheral Blood and Cancer Tissues of Non-small Cell Lung Cancer Patients

摘要

摘要:
目的探讨ZIC1基因在NSCLC患者外周血和肺肿瘤组织中的甲基化状态，及其对NSCLC的预后评估意义。
方法选取95例NSCLC患者外周血、癌和癌旁组织，另选取95例健康体检者外周血为对照组。用MSP法比较外周血和癌组织的ZIC1甲基化检出率；分析ZIC1在外周血和癌组织的甲基化和NSCLC临床病理因素的相关性。
结果 NSCLC患者的外周血和肺肿瘤组织中ZIC1甲基化检出率显著高于健康人群的外周血和NSCLC患者的癌旁组织(P < 0.05)，其中ZIC1甲基化在肿瘤组织诊断中的敏感度和特异性较高。NSCLC患者外周血和肿瘤组织ZIC1基因甲基化检出率与肿瘤直径、转移、分期及胸腔积液有显著相关性(P < 0.05)。
结论 ZIC1基因甲基化与NSCLC患者肿瘤发生、发展、转移、分期等有关，可能作为NSCLC诊断及预后指标。

Abstract:
Objective To investigate the methylation status of ZIC1 gene in peripheral blood and lung cancer tissues of NSCLC patients and its prognostic significance.
Methods We took the peripheral blood, cancer tissues and adjacent tissues of 95 NSCLC patients. The peripheral blood of 95 healthy people was taken as control group. MSP was used to compare the detection rate of ZIC1 methylation between peripheral blood and cancer tissues. And we analyzed the correlation of ZIC1 methylation in peripheral blood and cancer tissues with the clinicopathological factors of NSCLC patients.
Results The methylation detection rates of ZIC1 in peripheral blood and lung cancer tissues in NSCLC patients were significantly higher than those in peripheral blood of healthy people and adjacent tissues of NSCLC patients (P < 0.05), and the sensitivity and specificity of ZIC1 methylation in the diagnosis of tumor tissues were higher. The positive rate of ZIC1 gene methylation in peripheral blood and tumor tissues of NSCLC patients was significantly correlated with tumor diameter, metastasis, stage and pleural effusion (P < 0.05).
Conclusion The methylation of ZIC1 gene is related to the occurrence, development, metastasis and stage of NSCLC. It may be used as a diagnostic and prognostic indicator of NSCLC.

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