食管鳞癌患者外周血及癌组织中浸润性NK细胞表面受体的表达

王超; 杨晓燕; 高凤霞; 吴剑; 戴天阳

doi:10.3971/j.issn.1000-8578.2019.19.0027

食管鳞癌患者外周血及癌组织中浸润性NK细胞表面受体的表达

Expression of Infiltrating NK Cell Surface Receptors in Peripheral Blood and Cancer Tissues of Patients with Esophageal Squamous Cell Carcinoma

摘要

摘要:
目的分析食管鳞癌患者外周血及组织中NK细胞的比例及其表面受体的表达。
方法流式细胞仪检测食管鳞癌患者外周血及组织中NK细胞的比例及其表面受体的表达。
结果食管鳞癌组织中NK细胞比例明显高于癌旁5 cm以远的非肿瘤正常组织。CD16在食管鳞癌患者外周血中的表达与健康者外周血并无明显差异，但在鳞癌组织中的表达则明显低于正常组织；癌组织中NKp30、NKG2D的表达均明显低于正常组织；NKp44的表达明显高于正常组织。在同一患者体内，与外周血相比，癌组织中的CD16、NKp30、NKG2D的表达更低；而NKp44、NKG2A的表达更高。外周血与癌组织中浸润的NK细胞数量与肿瘤进展呈负相关。
结论食管鳞癌组织中NK细胞表面受体表达失衡可介导食管鳞癌细胞发生免疫逃逸。

Abstract:
Objective To analyze the ratio and the surface receptor expression of natural killer (NK) cells in peripheral blood and cancer tissues of esophageal squamous cell carcinoma (ESCC) patients.
Methods NK cell ratio and its surface receptor expression were detected by flow cytometry.
Results The ratio of NK cells in ESCC tissues was significantly higher than that in normal tissues. The expression of CD16 in peripheral blood of ESCC patients was not significantly different from that of healthy controls, however, that in cancer tissues was significantly lower than that in normal tissues. The expression of NKp30 and NKG2D in cancer tissues were significantly lower than those in normal tissues. The expression of NKp44 was significantly higher than that in normal tissues. In the same patient, the expression of CD16, NKp30 and NKG2D in cancer tissues were significantly lower while the expression of NKp44 and NKG2A were higher than those in peripheral blood. The number of infiltrating NK cells both in peripheral blood and cancer tissues were negatively correlated with the progression of tumor.
Conclusion The imbalance of NK cell surface receptor expression in ESCC tissues may mediate the immune escape of esophageal squamous cell carcinoma.

HTML全文

参考文献(20)

施引文献

资源附件(0)