lncRNA MEG3通过调控miR-543/PTEN分子轴抑制胰腺癌细胞增殖及转移的机制

成鉴晓; 江月萍; 任琳琳; 巩芮宁; 章程

doi:10.3971/j.issn.1000-8578.2019.18.1876

lncRNA MEG3通过调控miR-543/PTEN分子轴抑制胰腺癌细胞增殖及转移的机制

LncRNA MEG3 Suppresses Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating miR-543/PTEN Axis

摘要

摘要:
目的探讨lncRNA MEG3（MEG3）通过调控miR-543/PTEN分子轴介导胰腺癌细胞增殖和转移的分子机制。
方法采用qRT-PCR检测胰腺癌组织及细胞系中MEG3的表达水平；采用CCK-8、Transwell和Western blot探讨过表达MEG3对PANC-1细胞增殖、侵袭和上皮间质转化（EMT）的影响；采用双荧光素酶报告基因检测MEG3、miR-543及PTEN的靶向调控关系。进一步探讨MEG3通过调控miR-543/PTEN分子轴对PANC-1细胞增殖、侵袭和EMT的作用机制。
结果 MEG3在胰腺癌组织和细胞系中低表达。同时，过表达MEG3抑制PANC-1细胞增殖、侵袭和EMT。双荧光素酶报告基因和体外实验证实MEG3通过靶向下调miR-543对PTEN的抑制作用，进而抑制PANC-1细胞增殖、侵袭和EMT。
结论 MEG3通过调控miR-543/PTEN分子轴抑制PANC-1细胞增殖、侵袭和EMT，并可能为胰腺癌临床早期诊断和治疗提供潜在的分子靶点。

Abstract:
Objective To investigate the mechanism of lncRNA MEG3 (MEG3) modulating the proliferation and metastasis of pancreatic cancer cells via regulating miR-543/PTEN axis.
Methods qRT-PCR was applied to detect the expression of MEG3 in pancreatic cancer tissues and cell lines. The effect of MEG3 overexpression on the proliferation, invasion and epithelial mesenchymal transition (EMT) of PANC-1 cells were determined by CCK-8, Transwell assay and Western blot, respectively. The interaction between MEG3, miR-543 and PTEN were verified by dual-luciferase reporter gene assay. Furthermore, we investigated the effect of MEG3 on proliferation, invasion and EMT of PANC-1 cells through regulating miR-543/PTEN axis.
Results MEG3 expression was down-regulated in pancreatic cancer tissues and cell lines. Meanwhile, the overexpression of MEG3 inhibited the proliferation, invasion and EMT of PANC-1 cells in vitro. MEG3 suppressed the proliferation, invasion and EMT of PANC-1 cells via decreasing the inhibitory effect of miR-543 on PTEN in vitro.
Conclusion MEG3 inhibits the proliferation, invasion and EMT of PANC-1 cells by regulating miR-543/PTEN axis, which may provide potential molecular targets for early diagnosis and treatment of pancreatic cancer.

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